Using a Hamilton syringe, 200 nl of ATP was added (yield of 100 /xM) with either 1 p\ cAMP and/or the calculated ... The sample was viewed with a Nikon Optiphot microscope with a heated stage using either a 20x or 40x BM phase objective. ... For manual measurements, the viewed area on the microscope slide was recorded for 15 sec and changed to a new area during a total recording time of 5 min.
Title | : | Cellular regulators |
Author | : | Anthony R. Means, P. Michael Conn |
Publisher | : | - 1987-03-28 |
You must register with us as either a Registered User before you can Download this Book. You'll be greeted by a simple sign-up page.
Once you have finished the sign-up process, you will be redirected to your download Book page.
How it works: